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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for CDC25B Antibody (OAAF00676) |
|---|
| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Liquid |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot |
| Reconstitution and Storage | -20°C |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CDC25B. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity | CDC25B (Ab-323) antibody detects endogenous levels of total CDC25B protein. |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Dilution | The optimal working dilution should be determined by the end user. |
| Application Info | WB: 1:500~1:3000; IF: 1:100~1:500; ELISA: 1:20000 |
| Reference | Stéphanie Dutertre, J. Cell Sci., May 2004; 117: 2523 - 2531. Véronique Baldin, J. Biol. Chem., Dec 1997; 272: 32731. Estelle Schmitt, J. Cell Sci., Oct 2006; 119: 4269 - 4275. |
| Gene Symbol | CDC25B |
|---|---|
| Gene Full Name | cell division cycle 25B |
| Alias Symbols | CDC25 homolog B;dual specificity phosphatase Cdc25B;M-phase inducer phosphatase 2. |
| NCBI Gene Id | 994 |
| Protein Name | M-phase inducer phosphatase 2 |
| Description of Target | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. |
| Uniprot ID | P30305 |
| Protein Accession # | NP_004349.1 |
| Molecular Weight | 64 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CDC25B Antibody (OAAF00676)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "CDC25B Antibody (OAAF00676)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "CDC25B Antibody (OAAF00676)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CDC25B Antibody (OAAF00676)"?
This target may also be called "CDC25 homolog B;dual specificity phosphatase Cdc25B;M-phase inducer phosphatase 2." in publications.
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What is the shipping cost for "CDC25B Antibody (OAAF00676)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CDC25B Antibody (OAAF00676)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CDC25B Antibody (OAAF00676)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "64 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CDC25B Antibody (OAAF00676)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CDC25B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CDC25B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CDC25B"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CDC25B"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CDC25B"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CDC25B"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
