CDC25A ELISA Kit (Mouse) (OKCD08678)

Catalog No: OKCD08678

Size:96WELLS

CDC25A ELISA Kit (Mouse) (OKCD08678) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Division Cycle Protein 25 (CDC25). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cell Division Cycle Protein 25 (CDC25). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cell Division Cycle Protein 25 (CDC25), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cell Division Cycle Protein 25 (CDC25) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cell Division Cycle Protein 25 (CDC25) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cell Division Cycle Protein 25 (CDC25) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CDC25A Microplate: 96 Wells (12 x 8 Well strips)
  • CDC25A Lyophilized Standard: 2
  • 100X CDC25A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.055ng/mL

Target Info

• Gene Symbol: CDC25A
• Gene Full Name: cell division cycle 25A
• Alias Symbols: D9Ertd393, D9Ertd393e
• NCBI Gene Id: 12530
• Protein Name: M-phase inducer phosphatase 1
• Description of Target: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. directly dephosphorylates cdk1 and stimulates its kinase activity. also dephosphorylates cdk2 in complex with cyclin e, in vitro (by similarity). phosphorylation by pim1 leads to an increase in phosphatase activity (by similarity).
• Uniprot ID: P48964
• Protein Accession #: NP_031684.3
• Nucleotide Accession #: NM_007658.3