CDC16 Antibody (OAAF01101)

Catalog No: OAAF01101 (Formerly GWB-ASC500)

Size:100 ug

Western blot analysis of extracts from HuvEc cells, using CDC16/APC6 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CDC16/APC6 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human CDC16/APC6.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: CDC16/APC6 (Ab-560) antibody detects endogenous levels of total CDC16/APC6 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:5000
• Reference: Tugendreich S., Cell 81:261-268(1995).
  Ollendorff V., J. Biol. Chem. 272:32011-32018(1997).
  Kallio M., J. Cell Biol. 141:1393-1406(1998)

Target Info

• Gene Symbol: CDC16
• Gene Full Name: cell division cycle 16
• Alias Symbols: ANAPC6;Anaphase-promoting complex subunit 6;anaphase-promoting complex, subunit 6;APC6;CDC16 homolog;CDC16Hs;cell division cycle 16 homolog;cell division cycle protein 16 homolog;CUT9;cyclosome subunit 6.
• NCBI Gene Id: 8881
• Protein Name: Cell division cycle protein 16 homolog
• Description of Target: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
• Uniprot ID: Q13042
• Protein Accession #: NP_001072113.1
• Molecular Weight: 71 kDa