Cdc14 Antibody (OAAB17606)

Catalog No: OAAB17606

Size:400ul

His6-cdc14 Antibody (Cat. # OAAB17606) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the cdc14 antibody detected the cdc14 protein (arrow).

There is BioGPS gene expression data showing that CDC14A is expressed in HepG2

Cdc14 Antibody (Cat. # OAAB17606) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Cdc14 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Cdc14 Antibody (Cat. # OAAB17606) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Precautions: Cdc14 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Dual-specificity phosphatase. Required for centrosome separation and productive cytokinesis during cell division. May dephosphorylate the APC subunit FZR1/CDH1, thereby promoting APC- FZR1 dependent degradation of mitotic cyclins and subsequent exit from mitosis
  Cellular Location: Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Centrosomal during interphase, released into the cytoplasm at the onset of mitosis. Subsequently localizes to the midzone of the mitotic spindle
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Cdc14 antibody is generated from rabbits immunized with a recombinant protein encoding aa 1~379 of human cdc14.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that CDC14A is expressed in HepG2

• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: CDC14A
• Alias Symbols: cdc14, DFNB32, DFNB35, hCDC14, DFNB105
• NCBI Gene Id: 8556
• Protein Name: Dual specificity protein phosphatase CDC14A
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9UNH5
• Protein Accession #: NP_003663.2
• Molecular Weight: 66574 Da