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Catalog No: OKCA01541
Size:96 Wells
Price: $754.00
SKU
OKCA01541
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat9389-97
1:29692-100
1:4104100-112
1:89490-100
ELISA Kit PrincipleAviva Systems Biology CD96 ELISA Kit (Human) (OKCA01541) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD96 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD96 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CD96 captured in the well.
ELISA Kit Range0.156 - 10 ng/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)8984-93
EDTA Plasma (n=4)9388-97
ELISA Kit Component
ComponentAmount
CD96 Microplate96 Wells (12 x 8 Well strips)
CD96 Lyophilized Standard2 x 10 ng
100X Biotinylated CD96 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity0.039 ng/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolCD96
Gene Full NameCD96 molecule
Alias SymbolsTACTILE
NCBI Gene Id10225
Protein NameT-cell surface protein tactile
Description of TargetMay be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.
Uniprot IDP40200
Protein Accession #NP_005807.1
Nucleotide Accession #NM_005816.4
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