CD93 ELISA Kit (Human) (OKCD07382)

Catalog No: OKCD07382

Size:96WELLS

CD93 ELISA Kit (Human) (OKCD07382) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, Q Receptor (C1qR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 1, Q Receptor (C1qR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 1, Q Receptor (C1qR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Component 1, Q Receptor (C1qR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 1, Q Receptor (C1qR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 1, Q Receptor (C1qR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CD93 Microplate: 96 Wells (12 x 8 Well strips)
  • CD93 Lyophilized Standard: 2
  • 100X CD93 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 6.7pg/mL

Target Info

• Gene Symbol: CD93
• Gene Full Name: CD93 molecule
• Alias Symbols: C1q receptor 1;C1q/MBL/SPA receptor;C1qR;C1qR(P);C1QR1;C1qRP;CD93 antigen;CDw93;complement component 1 q subcomponent receptor 1;complement component C1q receptor;dJ737E23.1;ECSM3;matrix-remodeling-associated protein 4;matrix-remodelling associated 4;MXRA4.
• NCBI Gene Id: 22918
• Protein Name: Complement component C1q receptor
• Description of Target: Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion.
• Uniprot ID: Q9NPY3
• Protein Accession #: NP_036204.2
• Nucleotide Accession #: NM_012072.3