CD9 ELISA Kit (Mouse) (OKEH08299)

Catalog No: OKEH08299

Size:96T

Mouse CD9 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CD9 ELISA Kit (Mouse) (OKEH08299) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD9 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD9 captured in well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 116% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.0% (n = 20)
  Mean Inter-assay CV%: <=5.4% (n = 20)
• ELISA Kit Component:
  • CD9 Microplate: 96 Wells (12 x 8 Well strips)
  • CD9 Lyophilized Standard: 2
  • 100X Biotinylated CD9 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion (By similarity). Acts as a receptor for PSG17.
  Subcellular Location: Membrane Multi-pass membrane protein Cell membrane Multi-pass membrane protein Secreted Exosome
  Unigene: Mm.210676
  String: 10090.ENSMUSP00000032492
  KEGG: mmu:12527
  Pfam: PF00335
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 7.3pg/mL
• Specificity: Natural and recombinant Mouse CD9 antigen
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cd9
• Gene Full Name: CD9 antigen
• Alias Symbols: CD9 antigen;Tsp;Tspan29.
• NCBI Gene Id: 12527
• Protein Name: P40240
• Description of Target: CD9 antigen
• Uniprot ID: https://www.uniprot.org/uniprot/P40240
• Protein Accession #: NP_031683.1
• Nucleotide Accession #: NM_007657.3