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Datasheets/Manuals | Printable datasheet for OATA00413 |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey |
Product Format | Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2 |
Clonality | Monoclonal |
Clone | RPA-T8 |
Isotype | Mouse IgG1, kappa |
Host | Mouse |
Conjugation | PE: Phycoerythrin, CY7: Cyanine 7 |
Application | FC |
:: | Excitation Laser: Blue (488 nm), Green (532 nm), Yellow-Green (561 nm) |
:: | Emmision: 785 nm |
Reconstitution and Storage | 2-8C protected from light |
Specificity | CD8a |
Application Info | This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. |
Reference | Estes JD, Gordon SN, Zeng M, Chahroudi AM, Dunham RM, Staprans SI, Reilly CS, Silvestri G, and Haase AT. 2008. J. Immunol. 180: 6798-6807. (Flow cytometry - Rhesus macaque and Sooty Mangabey) Chlereth B, Fichtner I, Lorenczewski G, Kleindienst P, Brischwein K, da Silva A, Kufer P, Lutterbuese R, Junghahn I, Kasimir-Bauer S, Wimberger P, Kimmig R and Baeuerle PA. 2005. Cancer Res. 65: 2882-2889. (Immunohistochemistry - frozen tissue) Mack CL, Tucker RM, Sokol RJ, Darrer FM, Kotzin BL, Whitington PF and Miller SD. 2004. Pediatr. Res. 56(1) :79-87. (Immunohistochemistry - frozen tissue)Huang Z-Y, Hunter S, Kim M-K, Chien P, Worth RG, Indik ZK, and Schreiber AD. 2004. J. Leukoc. Biol. 76:491-499. (in vitro activation) Kayagaki N, Yamaguchi N, Nagao F, Matsuo S, Maeda H, Okumura K, and Yagita H. 1997. Proc. Natl. Acad. Sci. 94:3914-3919. (Immunoprecipitation - transfected cells) Deng MC, Bell S, Huie P, Pinto F, Hunt SA, Stinson EB, Sibley R, Hall BM, and Valantine HA. 1995. Circulation. 91: 1647-1654. (Immunohistochemistry - OCT embedded frozen tissue) |
Gene Symbol | CD8A |
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Gene Full Name | CD8a molecule |
Alias Symbols | CD8, p32, Leu2 |
NCBI Gene Id | 925 |
Protein Name | T-cell surface glycoprotein CD8 alpha chain |
Description of Target | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. |
Uniprot ID | P01732 |
Protein Accession # | NP_001139345.1 |
Nucleotide Accession # | NM_001145873.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CD8A Antibody : PE-Cy7 (OATA00413)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey".
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How long will it take to receive "CD8A Antibody : PE-Cy7 (OATA00413)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "CD8A Antibody : PE-Cy7 (OATA00413)" provided in?
This item is provided in "Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CD8A Antibody : PE-Cy7 (OATA00413)"?
This target may also be called "CD8, p32, Leu2" in publications.
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What is the shipping cost for "CD8A Antibody : PE-Cy7 (OATA00413)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CD8A Antibody : PE-Cy7 (OATA00413)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CD8A Antibody : PE-Cy7 (OATA00413)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CD8A Antibody : PE-Cy7 (OATA00413)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CD8A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CD8A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CD8A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CD8A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CD8A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CD8A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.