CD86 Antibody : APC (OATA00065)

Catalog No: OATA00065

C57Bl/6 splenocytes were unstimulated (left panel) or stimulated for 3 days with LPS (right panel) and stained with 0.06 ug APC Anti-Mouse CD86 (OATA00065) (solid line) or 0.06 ug APC Rat IgG2a isotype control (dashed line).

C57Bl/6 splenocytes were stimulated for 3 days with LPS and then stained with 0.06 ug APC Anti-Mouse CD86 (GL-1) manufactured by Aviva Systems Biology (left panel) or eBioscience (right panel).

Product Info

• Tested Species Reactivity: Mouse
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: GL-1 (GL1)
• Isotype: Rat IgG2a, kappa
• Host: Rat
• Conjugation: APC: Allophycocyanin
• Application: FC
  Excitation Laser: Red (633-640 nm)
  Emmision: 660 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD86 (B7-2)
• Application Info: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.
• Reference:

  Liu Z, Geboes K, Hellings P, Maerten P, Heremans H, Vandenberghe P, Boon L, van Kooten P, Rutgeerts P, and Ceuppens JL. 2011. J. Immunol. 167: 1830-1838. (in vivo blocking, Immunohistochemistry - OCT embedded frozen tissue)

  Kastenmuller W, Gasteiger G, Subramanian N, Sparwasser T, Busch DH, Belkaid Y, Drexler I, and Germain RN, 2011. J. Immunol. 187: 3186-3197. (in vivo blocking)

  Zheng SG, Wang JH, Stohl, W, Kim KS, Gray JD, and Horwitz DA. 2006. J. Immunol. 176:3321-3329. (in vitro blocking)

  Leithauser F, Meinhardt-Krajina T, Fink K, Wotschke B, Moller P and Reimann J. 2006. Am. J. Pathol. 168(6): 1898-1909. (Immunohistochemistry - frozen tissue)

  Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, and Holdsworth SR. 2005. J. Am. Soc. Nephrol. 16: 2012-2022. (in vivo activation, Immunofluorescence microscopy - frozen tissue, Immunohistochemistry - frozen tissue)

  Lenschow DJ, Ho SC, Sattar H, Rhee L, Gray G, Nabavi N,Herold KC, and Bluestone JA. 1995. J. Exp. Med. 181:1145-155. (in vitro blocking)

  Blazar BR, Taylor PA, Panoskaltsis-Mortari A, Gray GS, and Vallera DA. 1995. Blood. 85: 2607-2618. (Immunohistochemistry - OCT embedded frozen tissue)

Target Info

• Gene Symbol: CD86
• Gene Full Name: CD86 antigen
• Alias Symbols: B7, B70, Ly5, MB7, B7-2, B7.2, CLS1, Ly-5, Ly58, Cd28l, ETC-1, Ly-58, MB7-2, Cd28l2, TS/A-2
• NCBI Gene Id: 12524
• Protein Name: T-lymphocyte activation antigen CD86
• Uniprot ID: P42082
• Protein Accession #: NP_062261.3
• Nucleotide Accession #: NM_019388.3