CD83 ELISA Kit (Mouse) (OKCD04392)

Catalog No: OKCD04392

Size:96WELLS

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-104%	87-105%	90-101%	89-103%
  EDTA Plasma (n=5)	80-90%	97-104%	78-97%	80-103%
  Heparin Plasma (n=5)	83-101%	83-101%	83-92%	88-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 83 (CD83). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 83 (CD83). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 83 (CD83), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 83 (CD83) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 46.88-3,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	80-95	80
  EDTA Plasma (n=5)	83-96	88
  Heparin Plasma (n=5)	98-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 83 (CD83) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 83 (CD83) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Cd83 Microplate: 96 Wells (12 x 8 Well strips)
  • Cd83 Lyophilized Standard: 2
  • 100X Cd83 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 17.88 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cd83
• Gene Full Name: CD83 antigen
• Alias Symbols: CD83 antigen;mCD83.
• NCBI Gene Id: 12522
• Protein Name: CD83 antigen
• Description of Target: May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
• Uniprot ID: O88324
• Protein Accession #: NP_033986.1
• Nucleotide Accession #: NM_009856.3