CD83 ELISA Kit (Human) (OKEH08770)

Catalog No: OKEH08770

Size:96T

Human CD83 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	80-90% (n = 20)	96-105% (n = 20)	86-96% (n = 20)	89-99% (n = 20)
  EDTA plasma(n=5)	98-109% (n = 20)	108-117% (n = 20)	81-90% (n = 20)	87-96% (n = 20)
  heparin plasma(n=5)	103-112% (n = 20)	104-113% (n = 20)	103-112% (n = 20)	109-119% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology CD83 ELISA Kit (Human) (OKEH08770) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD83 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD83 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD83 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.2% (n = 20)
  Mean Inter-assay CV%: <=11.9% (n = 20)
• ELISA Kit Component:
  • CD83 Microplate: 96 Wells (12 x 8 Well strips)
  • CD83 Lyophilized Standard: 2
  • 100X Biotinylated CD83 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
  Subcellular Location: Membrane Single-pass type I membrane protein
  Unigene: Hs.595133
  SMR: Q01151
  String: 9606.ENSP00000368450
  KEGG: hsa:9308
  MIM: 604534
  Pfam: PF07686
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 37pg/mL
• Specificity: Natural and recombinant Human CD83 antigen
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CD83
• Gene Full Name: CD83 molecule
• Alias Symbols: B-cell activation protein;BL11;CD83 antigen;CD83 antigen (activated B lymphocytes, immunoglobulin superfamily);cell surface protein HB15;cell-surface glycoprotein;HB15;hCD83.
• NCBI Gene Id: 9308
• Protein Name: Q01151
• Description of Target: CD83 antigen
• Uniprot ID: https://www.uniprot.org/uniprot/Q01151
• Protein Accession #: NP_001035370.1
• Nucleotide Accession #: NM_001040280.1