CD82 Antibody (OAEE00321)

Catalog No: OAEE00321

Size:100UG

Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).
Commercially tested by LifeSpan BioSciences.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: C33
• Isotype: IgG2a
• Host: Mouse
• Application: Flow cytometry|Immunocytochemistry|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: C91/PL (human HTLV-1+ T cell line)
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody C33 reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Ueda T, Ichikawa T, Tamaru J, Mikata A, Akakura K, Akimoto S, Imai T, Yoshie O, Shiraishi T, Yatani R, Ito H, Shimazaki J: Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer. Am J Pathol. 1996 Nov;149(5):1435-40.
  Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles ofmultivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998 Aug 7;273(32):20121-7.
  Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1;155(3):1229-39.
  Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1;151(11):6470-81.
  Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. J Virol. 1992 Mar;66(3):1394-401.
  

Target Info

• Gene Symbol: CD82
• Gene Full Name: CD82 molecule
• Alias Symbols: 4F9;C33;C33 antigen;CD82 antigen;GR15;IA4;inducible membrane protein R2;KAI1;kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4));metastasis suppressor Kangai-1;R2;SAR2;ST6;Suppressor of tumorigenicity 6 protein;tetraspanin-27;tspan-27;TSPAN27.
• NCBI Gene Id: 3732
• Protein Name: CD82 antigen
• Description of Target: Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
• Uniprot ID: P27701
• Protein Accession #: NP_001020015.1; NP_002222.1; XP_006718286.1
• Nucleotide Accession #: NM_001024844.1; NM_002231.3; XM_006718223.1