Catalog No: OKEH02313
Size:96 Wells
Price: $525.00
SKU
OKEH02313
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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CD74 ELISA Kit (Human) : 96 Wells (OKEH02313)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CD74 ELISA Kit (Human) (OKEH02313) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD74 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD74 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD74 captured in well.
ELISA Kit Range0.625 - 40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 9.3% (n = 20)
ELISA Kit Component
ComponentAmount
CD74 Microplate96 Wells (12 x 8 Well strips)
CD74 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated NAGA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Human HLA class II histocompatibility antigen gamma chain
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCD74
Gene Full NameCD74 molecule
Alias SymbolsII, p33, DHLAG, HLADG, Ia-GAMMA
NCBI Gene Id972
Protein NameHLA class II histocompatibility antigen gamma chain
Description of TargetThe protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Swissprot IdP04233
Protein Accession #NP_001020329.1
Nucleotide Accession #NM_001025158.2
Protein Size (# AA)296
Molecular Weight34 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CD74.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CD74.
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