CD68 Polyclonal Antibody (OABB00472)

Catalog No: OABB00472 (Formerly GWB-BBP395)

Size:100ug

Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Lung Tissue Lysate

IHC(P): Rat Liver Tissue

Product Info

• Tested Species Reactivity: Mouse, Rat
• Predicted Species Reactivity: Hamster
• Product Format: Lyophilized 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na₂HPO₄
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: WB, IHC-P, IHC-F, IF
• Reconstitution and Storage: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse CD68(312-326aa AFCITRRRQSTYQPL), different from the related rat sequence by one amino acid.
• Purification: Immunogen affinity purified.
• Specificity: No cross reactivity with other proteins.
• Application Info: WB: 0.1-0.5 ug/mL, Rat and Mouse
  IHC-P: 0.5-1 ug/mL. Rat and Mouse
  IHC-F: 0.5-1 ug/mL. Mouse, Rat.
  IF 2 ug/mL, Rat.
• Publications:

  A Mitochondrial Specific Antioxidant Reverses Metabolic Dysfunction and Fatty Liver Induced by Maternal Cigarette Smoke in Mice. Nutrients. 11, (2019). 31330878

  Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from polyposis initiation. Int J Cancer. 138, 2700-12 (2016). 26773796

  Cellular senescence drives age-dependent hepatic steatosis. Nat Commun. 8, 15691 (2017). 28608850

  c-Rel orchestrates energy-dependent epithelial and macrophage reprogramming in fibrosis. Nat Metab. 2, 1350-1367 (2020) 33168981

  FPR-1 is an important regulator of neutrophil recruitment and a tissue-specific driver of pulmonary fibrosis. JCI Insight. 5, (2020). 32102985

  Moderate Exercise Inhibits Age-Related Inflammation, Liver Steatosis, Senescence, and Tumorigenesis. J Immunol. (2021). 33441438

  Moderate Exercise Inhibits Age-Related Inflammation, Liver Steatosis, Senescence, and Tumorigenesis. J Immunol. (2021). 31877385

  Rapamycin improves healthspan but not inflammaging in nfκb1-/- mice. Aging Cell. 18, e12882 (2019). 30468013

• Description: Rabbit IgG polyclonal antibody for Macrosialin(CD68) detection. Tested with WB, IHC-P, IHC-F, IF in Mouse;Rat.

Target Info

• Gene Symbol: CD68
• Gene Full Name: CD68 molecule
• Alias Symbols: CD 68, CD68, CD68 antigen, CD68 molecule, CD68_HUMAN, DKFZp686M18236, GP110, LAMP4, Macrophage antigen CD68(microsialin), MACROPHAGE ANTIGEN CD68, macrosialin, SCARD1, Scavenger receptor class D member 1
• Protein Name: Macrosialin
• Description of Target: CD68, cluster of differentiation, is a 110-kDa transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.
• Uniprot ID: P31996
• Molecular Weight: 35 kDa