Catalog No: OKEH03503
Price: $600.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CD68 ELISA Kit (Mouse) (OKEH03503) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd68 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cd68 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd68 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.2% (n = 20)
Mean Inter-assay CV%: <=8.6% (n = 20)
ELISA Kit Component
Cd68 Microplate96 Wells (12 x 8 Well strips)
Cd68 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Cd68 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.157 ng/mL
SpecificityNatural and recombinant Mouse Macrosialin

ACE2 activation protects against cognitive decline and reduces amyloid pathology in the Tg2576 mouse model of Alzheimer's disease. Acta Neuropathol. 139, 485-502 (2020). 31982938

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCd68
Gene Full NameCD68 antigen
Alias Symbolsgp11;gp110;La;Lamp4;ma;macrosialin;Sca;Scard1.
NCBI Gene Id12514
Protein NameP31996
Description of TargetMacrosialin
Uniprot IDhttps://www.uniprot.org/uniprot/P31996
Protein Accession #NP_001277987.1
Nucleotide Accession #NM_001291058.1
Protein Size (# AA)326
Molecular Weight35 kDa
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