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- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for Cd68 Antibody (OASA05368) |
|---|
| Predicted Species Reactivity | Mouse |
|---|---|
| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | FA-11 |
| Isotype | IgG2a |
| Host | Rat |
| Application | Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot |
| :: | Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant Preservative Stabilisers: 0.09% - Sodium Azide |
| :: | Approx Protein Conc: IgG concentration 1 mg/ml Buffer Solutions: Phosphate buffered saline pH7.4 |
| Reconstitution and Storage | -20°C |
| Immunogen | Purified Concanavalin A acceptor glycoprotein from P815 cell line. |
| Purification | Protein G Purified |
| Predicted Homology Based on Immunogen Sequence | Mouse |
| Concentration | 1 mg/ml |
| Specificity | CD68 |
| Application Info | Flow Cytometry(1): 1/50 - 1/100 |
| Application Data | Application #1: Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:Biotin .following permeablisation with Leucoperm (BUF09) Application #2: Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:FITC . following permeablisation with Leucoperm (BUF09)  Application #3: Staining of permeabilised mouse peritoneal macrophages cells with Rat anti Mouse CD68 visualised with F(ab')2 Goat anti Rat IgG:FITC (Mouse adsorbed)  Application #4: Staining of mouse peritoneal macrophages with Rat anti Mouse CD68 CD68 , following permeablisation with Leucoperm (BUF09)  Application #5: Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 . following permeablisation with Leucoperm (BUF09)  |
| Reference | 1. Ramprasad, M.P. et al. (1996) Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein. Proc. Natl. Acad. Sci. 93: 14833-14838. 2. Rabinowitz, S.S. & Gordon, S. (1991) Macrosialin, a macrophage-restricted membrane sialoprotein differentially glycosylated in response to inflammatory stimuli. J. Exp. Med. 174: 827-836. 3. Da Silva, R.P & Gordon, S. (1999) Phagocytosis stimulates alternative glycosylation of macrosialin (mouse CD68), a macrophage-specific endosomal protein. Biochem. J. 338: 687-694. 4. Schleicher, U. et al. (2005) Minute numbers of contaminant CD8+ T cells or CD11b+CD11c+ NK cells are the source of IFN-{gamma} in IL-12/IL-18-stimulated mouse macrophage populations. Blood 105: 1319-1328. 5. von Lukowicz, T. et al. (2008) PARP1 is required for adhesion molecule expression in atherogenesis. Cardiovasc. Res. 78: 158-166 |
| Gene Symbol | Cd68 |
|---|---|
| Alias Symbols | La, ma, Sca, gp11, Lamp4, gp110, Scard1 |
| NCBI Gene Id | 12514 |
| Protein Name | Macrosialin |
| Description of Target | RAT ANTI MOUSE CD68 |
| Uniprot ID | P31996 |
| Protein Accession # | NP_033983.1 |
| Protein Size (# AA) | 335 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Cd68 Antibody (OASA05368)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse".
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How long will it take to receive "Cd68 Antibody (OASA05368)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Cd68 Antibody (OASA05368)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Cd68 Antibody (OASA05368)"?
This target may also be called "La, ma, Sca, gp11, Lamp4, gp110, Scard1" in publications.
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What is the shipping cost for "Cd68 Antibody (OASA05368)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Cd68 Antibody (OASA05368)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Cd68 Antibody (OASA05368)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Cd68 Antibody (OASA05368)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CD68"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CD68"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CD68"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CD68"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CD68"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CD68"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
