Catalog No: OKEH01943 (Formerly GWB-TTD47C)
Size:96T
Price: $625.00
SKU
OKEH01943
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CD63 ELISA Kit (Human) : 96 Wells (OKEH01943)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH01943
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CD63 ELISA Kit (Human) (OKEH01943) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD63 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD63 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD63 captured in well.
ELISA Kit Range31.2-2000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 11% (n = 20)
ELISA Kit Component
ComponentAmount
CD63 Microplate96 Wells (12 x 8 Well strips)
CD63 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated COMT Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity19.5 pg/mL
SpecificityNatural and recombinant Human CD63 antigen
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCD63
Gene Full NameCD63 molecule
Alias SymbolsCD63 antigen;CD63 antigen (melanoma 1 antigen);granulophysin;LAMP-3;lysosomal-associated membrane protein 3;lysosome-associated membrane glycoprotein 3;ME491;melanoma-associated antigen ME491;melanoma-associated antigen MLA1;MLA1;ocular melanoma-associated antigen;OMA81H;tetraspanin-30;tspan-30;TSPAN30.
NCBI Gene Id967
Protein NameP08962
Description of TargetCD63 antigen
Uniprot IDhttps://www.uniprot.org/uniprot/P08962
Protein Accession #NP_001244318.1
Nucleotide Accession #NM_001257389.1
Protein Size (# AA)238
Molecular Weight26 kDa
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