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Datasheets/Manuals | Printable datasheet for CD63 Antibody (OAEE00946) (OAEE00946) |
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Predicted Species Reactivity | Human |
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Product Format | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Clonality | Monoclonal |
Clone | MEM-259 |
Isotype | IgG1 |
Host | Mouse |
Conjugation | FITC |
Application | Flow cytometry |
Reconstitution and Storage | 2°C to 8°C |
Immunogen | HPB-ALL T cell line |
Purification | Purified by size-exclusion chromatography |
Concentration | 20 ul / test |
Specificity | The antibody MEM-259 reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent. |
Formulation | FITC |
Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. |
Reference | Cerny J, Feng Y, Yu A, Miyake K, Borgonovo B, Klumperman J, Meldolesi J, McNeil PL, Kirchhausen T: The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing. EMBO Rep. 2004 Sep;5(9):883-8. Erratum in: EMBO Rep. 2005 Sep;6(9):898. Lin D, Kamsteeg EJ, Zhang Y, Jin Y, Sterling H, Yue P, Roos M, Duffield A, Spencer J, Caplan M, Wang WH: Expression of tetraspan protein CD63 activates protein tyrosine kinase (PTK) and enhances the PTK-induced inhibition of ROMK channels. J Biol Chem. 2008 Jan 22 Kwon MS, Shin SH, Yim SH, Lee KY, Kang HM, Kim TM, Chung YJ: CD63 as a biomarker for predicting the clinical outcomes in adenocarcinoma of lung. Lung Cancer. 2007 Jul;57(1):46-53. Israels SJ, McMillan-Ward EM: CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized fibrinogen. Thromb Haemost. 2005 Feb;93(2):311-8. Pfistershammer K, Majdic O, Stöckl J, Zlabinger G, Kirchberger S, Steinberger P, Knapp W: CD63 as an activation-linked T cell costimulatory element. J Immunol. 2004 Nov 15;173(10):6000-8. Mantegazza AR, Barrio MM, Moutel S, Bover L, Weck M, Brossart P, Teillaud JL, Mordoh J: CD63 tetraspanin slows down cell migration and translocates to theendosomal-lysosomal-MIICs route after extracellular stimuli in human immature dendritic cells. Blood. 2004 Aug 15;104(4):1183-90. Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM: LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004 Sep;61(1):62-8. |
Gene Symbol | CD63 |
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Gene Full Name | CD63 molecule |
Alias Symbols | CD63 antigen;CD63 antigen (melanoma 1 antigen);granulophysin;LAMP-3;lysosomal-associated membrane protein 3;lysosome-associated membrane glycoprotein 3;ME491;melanoma-associated antigen ME491;melanoma-associated antigen MLA1;MLA1;ocular melanoma-associated antigen;OMA81H;tetraspanin-30;tspan-30;TSPAN30. |
NCBI Gene Id | 967 |
Protein Name | CD63 antigen |
Description of Target | Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. |
Uniprot ID | P08962 |
Protein Accession # | NP_001244318.1; NP_001244319.1; NP_001244320.1; NP_001244321.1; NP_001244329.1; NP_001244330.1 |
Nucleotide Accession # | NM_001257; NM_001257389.1; NM_001257390.1; NM_001257391.1; NM_001257392.1; NM_001257400.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CD63 Antibody (OAEE00946)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "CD63 Antibody (OAEE00946)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "CD63 Antibody (OAEE00946)" provided in?
This item is provided in "Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CD63 Antibody (OAEE00946)"?
This target may also be called "CD63 antigen;CD63 antigen (melanoma 1 antigen);granulophysin;LAMP-3;lysosomal-associated membrane protein 3;lysosome-associated membrane glycoprotein 3;ME491;melanoma-associated antigen ME491;melanoma-associated antigen MLA1;MLA1;ocular melanoma-associated antigen;OMA81H;tetraspanin-30;tspan-30;TSPAN30." in publications.
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What is the shipping cost for "CD63 Antibody (OAEE00946)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CD63 Antibody (OAEE00946)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CD63 Antibody (OAEE00946)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CD63 Antibody (OAEE00946)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CD63"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CD63"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CD63"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CD63"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CD63"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CD63"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.