CD6 ELISA Kit (Human) (OKBB01532)

Catalog No: OKBB01532

Size:96 wells

CD6 ELISA Kit (Human) (OKBB01532) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: The Aviva Quick Picokine™ Human CD6 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human CD6 with immunogen: Expression system for standard: NS0; Immunogen sequence: H18-E398. To measure Human CD6, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CD6 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD6 in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Aviva's ELISA Resource Center at https://www.Avivabio.com/elisa-technical-resource-center.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	255	1450	4911	250	1511	5268
  Standard deviation	14.79	72.5	378.14	16.5	75.55	447.78
  CV(%)	5.8	5	7.7	6.6	5	8.5

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human CD6 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human CD6 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human CD6 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	255	257	218	221	237	18.29	7.7%
  Sample 2	1450	1421	1416	1269	1389	70.48	5%
  Sample 3	4911	4340	4503	4742	4624	218.85	4.7%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
  O.D.	0.002	0.108	0.219	0.416	0.728	1.202	1.845	2.546

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates,cell lysates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human CD6
• Immunogen: Expression system for standard: NS0; Immunogen sequence: H18-E398
• Description: The Quick version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human CD6 with <10 pg/ml sensitivity. Format: 96-well plate with removable strips.
  Compatible samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.

Target Info

• Gene Symbol: CD6
• Gene Full Name: T-cell differentiation antigen CD6
• Alias Symbols: T-cell differentiation antigen CD6;T12;TP120;CD6;CD6;
• NCBI Gene Id: 923
• Protein Name: T-cell differentiation antigen CD6
• Description of Target: CD6(Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich(SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What's more, this gene may be associated with susceptibility to multiple sclerosis.
• Uniprot ID: P30203
• Protein Accession #: NP_001241679.1
• Nucleotide Accession #: NM_001254750.1