CD59 ELISA Kit (Human) (OKCD07331)

Catalog No: OKCD07331

Size:96WELLS

CD59 ELISA Kit (Human) (OKCD07331) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster of Differentiation 59 (CD59). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster of Differentiation 59 (CD59). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster of Differentiation 59 (CD59), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster of Differentiation 59 (CD59) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 12.5-800pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster of Differentiation 59 (CD59) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster of Differentiation 59 (CD59) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CD59 Microplate: 96 Wells (12 x 8 Well strips)
  • CD59 Lyophilized Standard: 2
  • 100X CD59 HRP-Detector Anitbody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, cell lysates and other biological fluids
• Sensitivity: < 5.5pg/mL

Target Info

• Gene Symbol: CD59
• Gene Full Name: CD59 molecule (CD59 blood group)
• Alias Symbols: 16.3A5;1F5;1F5 antigen;20 kDa homologous restriction factor;CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344);CD59 blood group antigen;CD59 glycoprotein;CD59 molecule, complement regulatory protein;EJ16;EJ30;EL32;G344;HRF20;HRF-20;human leukocyte antigen MIC11;Ly-6-like protein;lymphocytic antigen CD59/MEM43;MACIF;MAC-inhibitory protein;MAC-IP;MEM43;MEM43 antigen;membrane attack complex (MAC) inhibition factor;membrane attack complex inhibition factor;membrane inhibitor of reactive lysis;MIC11;MIN1;MIN2;MIN3;MIRL;MSK21;p18-20;protectin;surface anitgen recognized by monoclonal antibody 16.3A5;T cell-activating protein.
• NCBI Gene Id: 966
• Protein Name: CD59 glycoprotein
• Description of Target: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
• Uniprot ID: P13987
• Protein Accession #: NP_000602.1
• Nucleotide Accession #: NM_000611.5