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| Datasheets/Manuals | Printable datasheet for CD59 Antibody (OASA04669) |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | YTH53.1 |
| Isotype | IgG2b |
| Host | Rat |
| Application | Flow cytometry|Functional assay|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot |
| Additional Information | Fusion Partners: Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma line. Histology Positive Control Tissue: Placenta |
| :: | Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant Preservative Stabilisers: 0.09% - Sodium Azide |
| :: | Approx Protein Conc: IgG concentration 1.0 mg/ml Buffer Solutions: Phosphate buffered saline |
| Reconstitution and Storage | -20°C |
| Immunogen | Human Peripheral Blood T cells. |
| Purification | Protein G Purified |
| Predicted Homology Based on Immunogen Sequence | Human |
| Concentration | 1 mg/ml |
| Specificity | CD59 |
| Application Info | Flow Cytometry: 1/50 - 1/100 Immunohistology - Paraffin(1): 1/5 - 1/10 /div> (1) This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. |
| Application Data | Application #1: Staining of human peripheral blood granulocytes with Rat anti Human CD59  |
| Reference | 1. Davies, A. et al. (1989) CD59 an LY-6-Like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. J. Exp. Med. 170: 637-654. 2. Walsh, L. A. et al. (1991) Transfection of Human CD59 complementary DNA into rat cells confers resistance to human complement. Eur. J. Immunol. 21: 847-850. 3. Davies, A. et al. (1995) Identification of MIC11 antigen as an epitope of the CD59 molecule. Immunology. 85: 220-227. 4. Ellison, B. S. et al. (2007) Complement susceptibility in glutamine deprived breast cancer cells. Cell Div. 11: 20-33. 5. Ajona, D. et al. (2007) Down-regulation of human complement factor H sensitizes non-small lung cancer cells to complement attack and reduces in vivo tumor growth. J. Immunol. 178: 5991-5998. |
| Gene Symbol | CD59 |
|---|---|
| Alias Symbols | 1F5, EJ16, EJ30, EL32, G344, MIN1, MIN2, MIN3, MIRL, HRF20, MACIF, MEM43, MIC11, MSK21, 16.3A5, HRF-20, MAC-IP, p18-20 |
| NCBI Gene Id | 966 |
| Protein Name | CD59 glycoprotein |
| Description of Target | RAT ANTI HUMAN CD59 |
| Uniprot ID | P13987 |
| Protein Accession # | NP_000602.1 |
| Protein Size (# AA) | 128 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CD59 Antibody (OASA04669)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "CD59 Antibody (OASA04669)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "CD59 Antibody (OASA04669)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CD59 Antibody (OASA04669)"?
This target may also be called "1F5, EJ16, EJ30, EL32, G344, MIN1, MIN2, MIN3, MIRL, HRF20, MACIF, MEM43, MIC11, MSK21, 16.3A5, HRF-20, MAC-IP, p18-20" in publications.
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What is the shipping cost for "CD59 Antibody (OASA04669)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CD59 Antibody (OASA04669)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CD59 Antibody (OASA04669)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CD59 Antibody (OASA04669)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CD59"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CD59"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CD59"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CD59"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CD59"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CD59"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
