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Datasheets/Manuals | Printable datasheet for CD59 Antibody (OAEE00287) (OAEE00287) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Clonality | Monoclonal |
Clone | MEM-43/5 |
Isotype | IgG2b |
Host | Mouse |
Application | Flow cytometry|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot |
:: | Regulatory Status: RUO |
Reconstitution and Storage | 2°C to 8°C |
Immunogen | Thymocytes and T lymphocytes |
Purification | Purified |
Concentration | 1 mg/ml |
Specificity | The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies. HLDA V; WS Code AS S012 |
Purity | > 95% (by SDS-PAGE) |
Application Info | WB: 1-2 ug/mL. Non-reducing conditions. Positive Control: mouse spleen FC: 1 ug/mL Positive Control: blood IHC-P: 5 ug/mL |
Reference | Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsi V, Waldhausl W: Signal transduction via glycosyl phosphatidylinositol-anchored proteins in Tcells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1;272(31):19242-7. Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schutz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84. Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). Bodian DL, Davis SJ, Morgan BP, Rushmere NK.: Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59. J Exp Med. 1997 Feb 3;185(3):507-16. Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP: CD59a is the primary regulator of membrane attack complex assembly in the mouse. J Immunol. 2004 Sep 15;173(6):3684-92. Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE: CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation. J Immunol. 1994 Sep 15;153(6):2444-56. Rooney IA, Davies A, Griffiths D, Williams JD, Davies M, Meri S, Lachmann PJ, Morgan BP: The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells. Clin Exp Immunol. 1991 Feb;83(2):251-6. Meri S, Morgan BP, Davies A, Daniels RH, Olavesen MG, Waldmann H, Lachmann PJ: Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor,inhibits C5b-8 catalysed insertion of C9 into lipid bilayers. Immunology. 1990 Sep;71(1):1-9. |
Gene Symbol | CD59 |
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Gene Full Name | CD59 molecule (CD59 blood group) |
Alias Symbols | 16.3A5;1F5;1F5 antigen;20 kDa homologous restriction factor;CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344);CD59 blood group antigen;CD59 glycoprotein;CD59 molecule, complement regulatory protein;EJ16;EJ30;EL32;G344;HRF20;HRF-20;human leukocyte antigen MIC11;Ly-6-like protein;lymphocytic antigen CD59/MEM43;MACIF;MAC-inhibitory protein;MAC-IP;MEM43;MEM43 antigen;membrane attack complex (MAC) inhibition factor;membrane attack complex inhibition factor;membrane inhibitor of reactive lysis;MIC11;MIN1;MIN2;MIN3;MIRL;MSK21;p18-20;protectin;surface anitgen recognized by monoclonal antibody 16.3A5;T cell-activating protein. |
NCBI Gene Id | 966 |
Protein Name | CD59 glycoprotein |
Description of Target | Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. |
Uniprot ID | P13987 |
Protein Accession # | NP_000602.1; NP_001120695.1; NP_001120697.1; NP_001120698.1; NP_001120699.1; NP_976074.1; NP_976075.1; NP_976076.1 |
Nucleotide Accession # | NM_000611.5; NM_001127223.1; NM_001127225.1; NM_001127226.1; NM_001127227.1; NM_203329.2; NM_203330.2; NM_203331.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CD59 Antibody (OAEE00287)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "CD59 Antibody (OAEE00287)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "CD59 Antibody (OAEE00287)" provided in?
This item is provided in "Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CD59 Antibody (OAEE00287)"?
This target may also be called "16.3A5;1F5;1F5 antigen;20 kDa homologous restriction factor;CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344);CD59 blood group antigen;CD59 glycoprotein;CD59 molecule, complement regulatory protein;EJ16;EJ30;EL32;G344;HRF20;HRF-20;human leukocyte antigen MIC11;Ly-6-like protein;lymphocytic antigen CD59/MEM43;MACIF;MAC-inhibitory protein;MAC-IP;MEM43;MEM43 antigen;membrane attack complex (MAC) inhibition factor;membrane attack complex inhibition factor;membrane inhibitor of reactive lysis;MIC11;MIN1;MIN2;MIN3;MIRL;MSK21;p18-20;protectin;surface anitgen recognized by monoclonal antibody 16.3A5;T cell-activating protein." in publications.
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What is the shipping cost for "CD59 Antibody (OAEE00287)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CD59 Antibody (OAEE00287)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CD59 Antibody (OAEE00287)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CD59 Antibody (OAEE00287)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CD59"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CD59"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CD59"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CD59"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CD59"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CD59"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.