CD58 ELISA Kit (Human) (OKCD06979)

Catalog No: OKCD06979

Size:96WELLS

CD58 ELISA Kit (Human) (OKCD06979) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 58 (CD58). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 58 (CD58). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 58 (CD58), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 58 (CD58) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 12.50-800pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 58 (CD58) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 58 (CD58) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CD58 Microplate: 96 Wells (12 x 8 Well strips)
  • CD58 Lyophilized Standard: 2
  • 100X CD58 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, cell lysatesand other biological fluids.
• Sensitivity: < 5.8pg/mL

Target Info

• Gene Symbol: CD58
• Gene Full Name: CD58 molecule
• Alias Symbols: ag3;CD58 antigen, (lymphocyte function-associated antigen 3);LFA3;LFA-3;lymphocyte function-associated antigen 3;surface glycoprotein LFA-3.
• NCBI Gene Id: 965
• Protein Name: Lymphocyte function-associated antigen 3
• Description of Target: Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.
• Uniprot ID: P19256
• Protein Accession #: NP_001138294.1
• Nucleotide Accession #: NM_001144822.1