CD56/NCAM1 ELISA Kit (Mouse) (OKBB00658)

Catalog No: OKBB00658

Size:96 Wells

Mouse CD56/NCAM1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD56 has been precoated onto 96-well plates. Standards(NS0, L20-T711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD56 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD56 amount of sample captured in plate.
• ELISA Kit Range: 625 pg/ml - 40,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	6.7	17.2	28.6	8.3	19.5	32.5
  SD	0.281	0.946	1.83	3.984	1.326	2.34
  CV%	4.2	5.5	6.4	4.8	6.8	7.2

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse CD56 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse CD56 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse CD56 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <20 pg/ml
• Specificity: Natural and recombinant mouse CD56
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: L20-T711
• Description: For quantitative detection of mouse CD56 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: NCAM1
• Gene Full Name: neural cell adhesion molecule 1
• Alias Symbols: CD56, NCAM 1, NCAM C, NCAM1, NCAM-1, N-CAM-1, P13591
• NCBI Gene Id: 17967
• Protein Name: Neural cell adhesion molecule 1
• Description of Target: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
• Uniprot ID: P13595
• Protein Accession #: NP_001074914.1
• Nucleotide Accession #: NM_001081445.1