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Catalog No: OKBB00309
Size:96 Tests
Price: $526.00
SKU
OKBB00309
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's human CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CD56 has been precoated onto 96-well plates. Standards(NS0, L20-G718) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD56 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CD56 amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (ng/mL)1.523.485.342.064.166.27
St. Dev.0.0820.2120.3680.140.3120.508
%CV5.396.096.896.797.58.10
ELISA Kit Component
ComponentAmount
Lyophilized recombinant human CD56/NCAM-1  standard10 ng/tube x 2
Anti-human CD56/NCAM-1a  Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti-human CD56/NCAM-1  antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Additional InformationRange: 78pg/ml-5000pg/ml
::Principle: Aviva’s human CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human CD56 specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD56 amount of sample captured in plate.
::Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
Predicted Homology Based on Immunogen SequenceNo detectable cross-reactivity with any other cytokine.
Reference1. Reyes AA, Small SJ, Akeson R. (1991). "At least 27 alternatively spliced forms of the neural cell adhesion molecule
mRNA are expressed during rat heart development". Mol Cell Biol. 11 (3): 1654–61. PMC 369464. PMID 1996115.
2. Nguyen, C., Mattei, M. G., Mattei, J.-F., Santoni, M.-J., Goridis, C., Jordan, B. R. Localization of the human NCAM
gene to band q23 of chromosome 11: the third gene coding for a cell interaction molecule mapped to the distal portion
of the long arm of chromosome 11. J. Cell Biol. 102: 711-715, 1986. [PubMed: 2869046, related citations] [Full Text:
HighWire Press, Pubget].
SpecificityNatural and recombinant human CD56
ImmunogenExpression system for standard: NS0; Immunogen sequence: L20-G718
DescriptionFor quantitative detection of human CD56 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolNCAM1
Gene Full Nameneural cell adhesion molecule 1
Alias SymbolsCD56, NCAM 1, NCAM C, NCAM1, NCAM-1, N-CAM-1, P13591
NCBI Gene Id4684
Protein NameNeural cell adhesion molecule 1
Description of TargetNeural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
Uniprot IDP13591
Protein Accession #NP_000606.3
Nucleotide Accession #NM_000615.6
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