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CD53 Antibody (OASA02226)

  • Catalog#: OASA02226
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Gene Symbol:
CD53
NCBI Gene Id:
963
Protein Name:
Leukocyte surface antigen CD53
Swissprot Id:
P19397
Protein Accession #:
NP_000551.1
Alias Symbols:
MOX44, TSPAN25, CD53
Replacement Item:
This antibody may replace item sc-21778 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI HUMAN CD53
Protein Size (# AA):
219
Host:
Mouse
Clonality:
Monoclonal
Application:
IHC-AFF, FC, IP, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD53.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD53.
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-CD53 antibody - OASA02226
Specificity:
CD53
Clone:
MEM-53
Isotype:
IgG1
Application Info:
Flow Cytometry:    1/10 - 1/25
Western Blotting(1):    Non-reducing conditions
(1)See [*] for special conditions.
Application Data:
Application #1: Staining of human peripheral blood granulocytes with Mouse anti Human CD53
Application #2: Staining of human peripheral blood monocytes with Mouse anti Human CD53:FITC
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G
Preservative Stabilisers: 0.09% - Sodium Azide
:::
Approx Protein Conc: IgG concentration 1 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Angelisova, P. et al. (1990) The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics 32: 281-285.
2. Bazil, V. et al. (1989) Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102 Fol. Biol. (Prague) 35: 289-98.

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