CD53 Antibody (OAEE00272)

Catalog No: OAEE00272

Size:0.1 mg

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: MEM-53
• Isotype: IgG1
• Host: Mouse
• Application: FC, IP, WB, IHC-F, FN
• Reconstitution and Storage: Store at 2-8C. Do not freeze. Do not use after expiration date stamped on vial label.
• Immunogen: Leukocytes of pacient suffering from a LGL-type leukemia.
• Purification: Purified by protein A
• Concentration: 1 mg/ml
• Specificity: The antibody MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells.
  The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
  HLDA IV; WS Code NL 59
  HLDA V; WS Code B CD53.5
  HLDA V; WS Code BP BP287
  HLDA V; WS Code T T-096
  HLDA V; WS Code X XB004
• Formulation: Azide free
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized.
• Reference: Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1;85(9):2402-13.
  Szollosi J, Horejsi V, Bene L, Angelisova P, Damjanovich S: Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol. 1996 Oct 1;157(7):2939-46.
  Rasmussen AM, Blomhoff HK, Stokke T, Horejsi V, Smeland EB: Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol. 1994 Dec 1;153(11):4997-5007.
  Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha). 1989;35(5):289-97.
  Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  Olweus J, Lund-Johansen F, Horejsi V: CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol. 1993 Jul 15;151(2):707-16.
  Angelisova P, Vlcek C, Stefanova I, Lipoldova M, Horejsi V: The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens.Immunogenetics. 1990;32(4):281-5.
  Yunta M, Rodríguez-Barbero A, Arévalo MA, López-Novoa JM, Lazo PA: Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primarycultures of mesangial cells. Kidney Int. 2003 Feb;63(2):534-42.
  Yunta M, Lazo PA: Apoptosis protection and survival signal by the CD53 tetraspanin antigen. Oncogene. 2003 Feb 27;22(8):1219-24.
  Kim TR, Yoon JH, Kim YC, Yook YH, Kim IG, Kim YS, Lee H, Paik SG: LPS-induced CD53 expression: a protection mechanism against oxidative and radiation stress. Mol Cells. 2004 Feb 29;17(1):125-31.
  Puls KL, Hogquist KA, Reilly N, Wright MD: CD53, a thymocyte selection marker whose induction requires a lower affinity TCR-MHC interaction than CD69, but is up-regulated with slower kinetics. Int Immunol. 2002 Mar;14(3):249-58.
  Mollinedo F, Martín-Martín B, Gajate C, Lazo PA: Physiological activation of human neutrophils down-regulates CD53 cell surface antigen. J Leukoc Biol. 1998 Jun;63(6):699-706.

Target Info

• Gene Symbol: CD53
• Gene Full Name: Leukocyte surface antigen CD53
• Alias Symbols: MOX44, TSPAN25
• NCBI Gene Id: 963
• Protein Name: Leukocyte surface antigen CD53
• Uniprot ID: P19397
• Protein Accession #: NP_000551.1; NP_001035122.1
• Nucleotide Accession #: NM_000560.3; NM_001040033.1