Catalog No: OKEH04460 (Formerly GWB-U9F2Q6)
Size:96T
Price: $525.00
SKU
OKEH04460
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04460
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CD52 ELISA Kit (Human) (OKEH04460) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD52 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CD52 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD52 captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.2% (n = 20)
Mean Inter-assay CV%: <= 9.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CD52 Microplate96 Wells (12 x 8 Well Strips)
CD52 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
CD52 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human CAMPATH-1 antigen
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCD52
Gene Full NameCD52 molecule
Alias Symbolscambridge pathology 1 antigen;CAMPATH-1 antigen;CD52 antigen (CAMPATH-1 antigen);CDW52;CDW52 antigen (CAMPATH-1 antigen);EDDM5;epididymal secretory protein E5;epididymis secretory sperm binding protein Li 171mP;HE5;HEL-S-171mP;human epididymis-specific protein 5.
NCBI Gene Id1043
Protein NameP31358
Description of TargetCAMPATH-1 antigen
Uniprot IDhttps://www.uniprot.org/uniprot/P31358
Protein Accession #NP_001794.2
Nucleotide Accession #NM_001803.2
Protein Size (# AA)61
Molecular Weight7 KDa
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