- NCBI Gene Id:
- Protein Name:
- CAMPATH-1 antigen
- Alias Symbols:
- CDW52, CD52
- Replacement Item:
- This antibody may replace item sc-25838 from Santa Cruz Biotechnology.
- Description of Target:
- RAT ANTI HUMAN CD52
- Protein Size (# AA):
- IHC-AFF, CT, ELISA, FC, IP, IHC-FFPE, IHC-R, WB
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express CD52.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express CD52.
- Predicted Species Reactivity:
- Predicted Homology Based on Immunogen Sequence:
- Product Format:
- Purified IgG - liquid
- Reconstitution and Storage:
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for anti-CD52 antibody - OASA04660
- Application Info:
- Flow Cytometry: 1/20
Immunohistology - Frozen: 1/20
Immunohistology - Paraffin(1): 1/20 - 1/40
Cytotoxic Assays: 50ug/ml (Use human serum as complement source)
(1)Special conditions apply. - See [*].
- Application Data:
- Application #1: Staining of human peripheral blood lymphocytes with Rat anti Human CD52: Alexa Fluor 647
Application #2: Staining of human peripheral blood lymphocytes with Rat anti Human CD52: Low Endotoxin
Application #3: Staining of human peripheral blood lymphocytes with Rat anti Human CD52:Biotin
Application #4: Staining of human peripheral blood lymphocytes with Rat anti Human CD52:FITC
Application #5: Staining of human peripheral blood lymphocytes with Rat anti Human CD52:RPE
- Additional Information:
- Histology Positive Control Tissue: Tonsil
- Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
- Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline
- Target Reference:
- 1. Hale, G. et al. (1998) Improving the Outcome of Bone Marow Transplantation by using CD52 Monoclonal Antibodies to prevent graft-versus-host disease and graft rejection. Blood 92: 4581-4590.
2. Salisbury, J.R. et al. (1994) Immunohistochemical analysis of CDw52 antigen expression in non-hodgkins lymphomas. J. Clin. Path. 47: 313-317.
3. Rodig, S. et al. (2006) Heterogeneous CD52 expression among hematologic neoplasms: implications for the use of alemtuzumab (CAMPATH-1H). Clin. Cancer Res. 12: 7174-7179.