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CD52 Antibody (OASA04660)

0.2 mg
$820.00
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Gene Symbol:
CD52
NCBI Gene Id:
1043
Protein Name:
CAMPATH-1 antigen
Swissprot Id:
P31358
Protein Accession #:
NP_001794.2
Alias Symbols:
CDW52, CD52
Replacement Item:
This antibody may replace item sc-25838 from Santa Cruz Biotechnology.
Description of Target:
RAT ANTI HUMAN CD52
Protein Size (# AA):
61
Host:
Rat
Clonality:
Monoclonal
Application:
IHC-AFF, CT, ELISA, FC, IP, IHC-FFPE, IHC-R, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD52.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD52.
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-CD52 antibody - OASA04660
Specificity:
CD52
Clone:
YTH34.5
Isotype:
IgG2b
Application Info:
Flow Cytometry:    1/20
Immunohistology - Frozen:    1/20
Immunohistology - Paraffin(1):    1/20 - 1/40
Cytotoxic Assays:    50ug/ml (Use human serum as complement source)
(1)Special conditions apply. - See [*].
Application Data:
Application #1: Staining of human peripheral blood lymphocytes with Rat anti Human CD52: Alexa Fluor 647
Application #2: Staining of human peripheral blood lymphocytes with Rat anti Human CD52: Low Endotoxin
Application #3: Staining of human peripheral blood lymphocytes with Rat anti Human CD52:Biotin
Application #4: Staining of human peripheral blood lymphocytes with Rat anti Human CD52:FITC
Application #5: Staining of human peripheral blood lymphocytes with Rat anti Human CD52:RPE
Additional Information:
Histology Positive Control Tissue: Tonsil
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline
Target Reference:
1. Hale, G. et al. (1998) Improving the Outcome of Bone Marow Transplantation by using CD52 Monoclonal Antibodies to prevent graft-versus-host disease and graft rejection. Blood 92: 4581-4590.
2. Salisbury, J.R. et al. (1994) Immunohistochemical analysis of CDw52 antigen expression in non-hodgkins lymphomas. J. Clin. Path. 47: 313-317.
3. Rodig, S. et al. (2006) Heterogeneous CD52 expression among hematologic neoplasms: implications for the use of alemtuzumab (CAMPATH-1H). Clin. Cancer Res. 12: 7174-7179.

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