CD5 Antibody : APC (OATA00023)

Catalog#: OATA00023

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Capuchin Monkey, Chimpanzee, Common Marmoset
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: UCHT2
• Isotype: Mouse IgG1, kappa
• Host: Mouse
• Conjugation: APC: Allophycocyanin
• Application: FC
  Excitation Laser: Red (633-640 nm)
  Emmision: 660 nm
• Reconstitution and Storage: 2-8C protected from light
• Gene Symbol: CD5
• NCBI Gene Id: 921
• Official Gene Full Name: CD5 molecule
• Protein Name: T-cell surface glycoprotein CD5
• Swissprot Id: P06127
• Protein Accession #: NP_001333385.1
• Nucleotide Accession #: NM_001346456.1
• Alias Symbols: T1, LEU1
• Description of Target: This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms.
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express CD5.
• Specificity: CD5
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
• Target Reference:

  Knapp W, Dorken B, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

  Luo W, Van de Velde H, von Hoegen I, Parnes JR, Thielemans K. 1992. J Immunol. 148(6): 1630-1634.

  Tarakhovsky A, Kanner SB, Hombach J, et al. 1995. Science. 269(5223): 535-537.

  Wood GS and Freudenthal PS. 1992. Am J Pathol. 141(4): 789-795. (Flow Cytometry)

Protocol:

Tips Information: