CD47 Antibody (OASA02114)

Catalog No: OASA02114 (Formerly GWB-D297F3)

Size:25UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. TRIS buffered glycine
• Clonality: Monoclonal
• Clone: BRIC126
• Isotype: IgG2b
• Host: Mouse
• Application: Flow cytometry|Functional assay|Immunohistochemistry-Frozen|Immunoprecipitation|Western blot
  Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
  Preservative Stabilisers: 0.09% - Sodium Azide
  Approx Protein Conc: IgG concentration 1 mg/ml
• Reconstitution and Storage: -20°C
• Immunogen: Human erythrocytes
• Purification: Protein G Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Specificity: This product recognizes the human CD47 cell surface glycoprotein, a heavily N­glycosylated 47­52kD molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.
• Application Info: Flow Cytometry: 1/10
  
• Reference: 1. Avent, N.D. et al. (1989) Monoclonal antibodies that recognize different membrane proteins that are deficient in Rhnull human erythrocytes. One group of antibodies reacts with a variety of cells and tissues whereas the other group is erythroid-specific Biochem. J. 251: 499-505.
  2. Campbell, I.G. et al. (1992) An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains Cancer Res. 52: 5416 - 5420
  3. Brown, E. et al. (1990) Integrin-associated protein: a 50-kD plasma membrane antigen physically and functionally associated with integrins J. Cell Biol. 111: 2785.
  4. Lindberg, F.P. et al. (1993) Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding J. Cell Biol. 123: 485 - 496
  5. Ide, K. et al. (2007) Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages. Proc Natl Acad Sci U S A.104: 5062-6.
  6. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes

Target Info

• Gene Symbol: CD47
• Alias Symbols: IAP, OA3, MER6
• NCBI Gene Id: 961
• Protein Name: Leukocyte surface antigen CD47
• Description of Target: MOUSE ANTI HUMAN CD47
  
• Uniprot ID: Q08722
• Protein Accession #: NP_001020250.1
• Protein Size (# AA): 312