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Datasheets/Manuals | Printable datasheet for CD47 Antibody (OAEE00896) (OAEE00896) |
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Predicted Species Reactivity | Human|Non-human Primate|Porcine |
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Product Format | Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
Clonality | Monoclonal |
Clone | MEM-122 |
Isotype | IgM |
Host | Mouse |
Conjugation | FITC |
Application | Flow cytometry |
Reconstitution and Storage | 2°C to 8°C |
Immunogen | COS-7 (African green monkey) cells |
Purification | Purified by size-exclusion chromatography |
Concentration | 20 ul / test |
Specificity | The antibody MEM-122 reacts with CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. HLDA VI; WS Code AS A051 |
Formulation | FITC |
Storage Buffer | The reagent is provided in Tris buffered saline (TBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. |
Reference | Smith RE, Patel V, Seatter SD, Deehan MR, Brown MH, Brooke GP, Goodridge HS, Howard CJ, Rigley KP, Harnett W, Harnett MM: A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes. Blood. 2003 Oct 1;102(7):2532-40. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). Isenberg JS, Pappan LK, Romeo MJ, Abu-Asab M, Tsokos M, Wink DA, Frazier WA, Roberts DD: Blockade of thrombospondin-1-CD47 interactions prevents necrosis of full thickness skin grafts. Ann Surg. 2008 Jan;247(1):180-90. Orazizadeh M, Lee HS, Groenendijk BC, Millward-Sadler J, Wright MO, Lindberg FP, Salter DM. CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model. Arthritis Res Ther. 2008 Jan 10;10(1):R4 Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages. Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5062-6. Murata T, Ohnishi H, Okazawa H, Murata Y, Kusakari S, Hayashi Y, Miyashita M, Itoh H, Oldenborg PA, Furuya N, Matozaki T: CD47 promotes neuronal development through Src- and FRG/Vav2-mediated activationof Rac and Cdc42. J Neurosci. 2006 Nov 29;26(48):12397-407. Barazi HO, Li Z, Cashel JA, Krutzsch HC, Annis DS, Mosher DF, Roberts DD: Regulation of integrin function by CD47 ligands. Differential effects on alphavbeta 3 and alpha 4beta1 integrin-mediated adhesion. J Biol Chem. 2002 Nov 8;277(45):42859-66. |
Gene Symbol | CD47 |
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Gene Full Name | CD47 molecule |
Alias Symbols | antigen identified by monoclonal antibody 1D8;antigenic surface determinant protein OA3;CD47 antigen (Rh-related antigen, integrin-associated signal transducer);CD47 glycoprotein;IAP;integrin associated protein;Integrin-associated protein;integrin-associated signal transducer;leukocyte surface antigen CD47;MER6;OA3;Protein MER6;Rh-related antigen. |
NCBI Gene Id | 961 |
Protein Name | Leukocyte surface antigen CD47 |
Description of Target | Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. |
Uniprot ID | Q08722 |
Protein Accession # | NP_001768.1; NP_942088.1 |
Nucleotide Accession # | NM_001777.3; NM_198793.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CD47 Antibody (OAEE00896)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Non-human Primate|Porcine".
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How long will it take to receive "CD47 Antibody (OAEE00896)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "CD47 Antibody (OAEE00896)" provided in?
This item is provided in "Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CD47 Antibody (OAEE00896)"?
This target may also be called "antigen identified by monoclonal antibody 1D8;antigenic surface determinant protein OA3;CD47 antigen (Rh-related antigen, integrin-associated signal transducer);CD47 glycoprotein;IAP;integrin associated protein;Integrin-associated protein;integrin-associated signal transducer;leukocyte surface antigen CD47;MER6;OA3;Protein MER6;Rh-related antigen." in publications.
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What is the shipping cost for "CD47 Antibody (OAEE00896)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CD47 Antibody (OAEE00896)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CD47 Antibody (OAEE00896)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CD47 Antibody (OAEE00896)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CD47"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CD47"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CD47"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CD47"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CD47"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CD47"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.