CD46 ELISA Kit (Human) (OKCD07268)

Catalog No: OKCD07268

Size:96WELLS

CD46 ELISA Kit (Human) (OKCD07268) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Membrane Cofactor Protein (MCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Membrane Cofactor Protein (MCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Membrane Cofactor Protein (MCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Membrane Cofactor Protein (MCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 12.5-800pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Membrane Cofactor Protein (MCP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Membrane Cofactor Protein (MCP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CD46 Microplate: 96 Wells (12 x 8 Well strips)
  • CD46 Lyophilized Standard: 2
  • 100X CD46 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, urine, saliva and other biological fluids
• Sensitivity: < 4.8pg/mL

Target Info

• Gene Symbol: CD46
• Gene Full Name: CD46 molecule
• Alias Symbols: AHUS2;antigen identified by monoclonal antibody TRA-2-10;CD46 antigen, complement regulatory protein;CD46 molecule, complement regulatory protein;complement membrane cofactor protein;MCP;measles virus receptor;membrane cofactor protein;membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen);MIC10;TLX;TRA2.10;trophoblast leucocyte common antigen;trophoblast leukocyte common antigen;trophoblast-lymphocyte cross-reactive antigen.
• NCBI Gene Id: 4179
• Protein Name: Membrane cofactor protein
• Description of Target: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
• Uniprot ID: P15529
• Protein Accession #: NP_002380.3
• Nucleotide Accession #: NM_002389.4