CD46 Antibody (OASA02106)

Catalog No: OASA02106 (Formerly GWB-275C71)

Size:25UG

Staining of human peripheral blood lymphocytes with Mouse anti Human CD46:FITC

Staining of human peripheral blood granulocytes with Mouse anti Human CD46: Alexa Fluor 647

Staining of human peripheral blood lymphocytes with Mouse anti Human CD46

Staining of human peripheral blood lymphocytes with Mouse anti Human CD46:RPE

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Phosphate buffered saline
• Clonality: Monoclonal
• Clone: MEM-258
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunofluorescence|Immunoprecipitation|Western blot
  Preparation: Purified IgG prepared by affinity chromatography on Protein A
  Preservative Stabilisers: 0.09% - Sodium Azide
  Approx Protein Conc: IgG concentration 1.0 mg/ml
  Buffer Solutions: Phosphate buffered saline
• Reconstitution and Storage: -20°C
• Immunogen: HPB-ALL cell line.
• Purification: Protein A Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Specificity: CD46
• Application Info: Flow Cytometry:    1/10 - 1/50
  Western Blotting:    Non reducing conditions
  
• Application Data: Application #1: Staining of human peripheral blood lymphocytes with Mouse anti Human CD46:FITC
  Application #2: Staining of human peripheral blood granulocytes with Mouse anti Human CD46: Alexa Fluor 647
  Application #3: Staining of human peripheral blood lymphocytes with Mouse anti Human CD46
  Application #4: Staining of human peripheral blood lymphocytes with Mouse anti Human CD46:RPE
• Protocol Information: Species: Human
  Experiment Name: Gel electrophoresis, immunoblotting and phase separation in Triton X-114
  Experiment Background: 1. The aim of this study was to examine the nature of the 60 kDa MCP protein present in seminal plasma by sequential centrifugation and phase separation in the detergent Triton X-114.2. In this study K. L. Simpson and C. H. Holmes set out to investigate MCP proteins in human seminal plasma further. They confirmed that seminal plasma contains an MCP protein of approximately 60 kDa and demonstrated that seminal plasma may also contain a 50-55 kDa MCP component. MCP proteins were depleted from seminal plasma by ultracentrifugation and partitioned to the detergent phase when extracted with Triton X-114, suggesting that these products are membrane-associated. They show that seminal plasma from vasectomized donors contains MCP products with similar separation characteristics in Triton X-114 and that seminal plasma MCP is therefore derived distal to the ductus deferens.
  Experimental Steps: 1. Western blotting was carried out using a modification of the method described by Towbin et al (1979). 2. Membrane preparations and pellet and supernatant fractions derived from semen were subjected to SDS-PAGE under nonreducing conditions on gels containing 10% (w/v) acrylamide according to the method of Laemmli (1970) and electro-transferred to Immobilon-P polyvinylidene difluoride membrane (Millipore, UK Ltd., Watford). 3. The membranes were blocked with 5% (w/v) dried milk powder in PBS containing 0.2% (v/v) Tween 20, incubated for 2 h at room temperature with primary mAb, and mAb binding detected using an enhanced immunoperoxidase technique (Wainwright and Holmes, 1993). 4. Membrane preparations of testis and prostate, and supernatant and pellet fractions of semen were separated into detergent and aqueous phases by condensation in Triton X-114 as described by Bordier (1981).
  Other Reagents Used: Phenylmethyl sulfonyl fluoride
  Number Of Protocols: 3
• Reference: 1. Sirena, D. et al. (2004) The human membrane cofactor CD46 is a receptor for species B adenovirus serotype 3. J. Virol. 78:4454-4462.
  2. Fremeaux-Bacchi. et al. (2006) Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical Hemolytic Uremic Syndrome. J. Am. Soc. Nephrol. 17:2017-2025.
  3. Fleischli, C. et al. (2005) The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35. J Virol. 79:10013-22.
  4. Sweigard, J.H. et al. (2010) Adenovirus vectors targeting distinct cell types in the retina. Invest Ophthalmol Vis Sci. 51:2219-28.
  

Target Info

• Gene Symbol: CD46
• Alias Symbols: MCP, TLX, AHUS2, MIC10, TRA2.10
• NCBI Gene Id: 4179
• Protein Name: Membrane cofactor protein
• Description of Target: MOUSE ANTI HUMAN CD46
  
• Uniprot ID: P15529
• Protein Accession #: NP_002380.3
• Protein Size (# AA): 392