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Catalog No: OKEH03180
Size:96T
Price: $525.00
SKU
OKEH03180
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH03180
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CD44 ELISA Kit (Rat) (OKEH03180) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd44 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cd44 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd44 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Component
ComponentAmount
Anti-Cd44 Microplate96 Wells (12 x 8 Well strips)
Cd44 Lyophilized Standard2
100X Biotinylated Cd44 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.166 ng/mL
SpecificityNatural and recombinant Rat CD44 antigen
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCd44
Gene Full NameCD44 molecule (Indian blood group)
Alias SymbolsCD44 antigen;CD44A;Cell surface glycoprotein CD44 (hyaluronate binding protein);ECMR-III;extracellular matrix receptor III;GP90 lymphocyte homing/adhesion receptor;hermes antigen;HUTCH-I;hyaluronate receptor;METAA;PGP-1;PGP-I;phagocytic glycoprotein 1;phagocytic glycoprotein I;RHAMM.
NCBI Gene Id25406
Protein NameP26051
Description of TargetCD44 antigen
Uniprot IDhttps://www.uniprot.org/uniprot/P26051
Protein Accession #NP_037056.2
Nucleotide Accession #NM_012924.2
Protein Size (# AA)780
Molecular Weight86 kDa
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