CD44 ELISA Kit (Mouse) (OKCD06645)

Catalog No: OKCD06645

Size:96WELLS

CD44 ELISA Kit (Mouse) (OKCD06645) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Homing Associated Cell Adhesion Molecule (HCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Homing Associated Cell Adhesion Molecule (HCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Homing Associated Cell Adhesion Molecule (HCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Homing Associated Cell Adhesion Molecule (HCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Homing Associated Cell Adhesion Molecule (HCAM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Homing Associated Cell Adhesion Molecule (HCAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CD44 Microplate: 96 Wells (12 x 8 Well strips)
  • CD44 Lyophilized Standard: 2
  • 100X CD44 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 3.4pg/mL

Target Info

• Gene Symbol: Cd44
• Gene Full Name: CD44 antigen
• Alias Symbols: AU023126;AW121933;AW146109;CD44 antigen;ECMR-III;extracellular matrix receptor III;GP90 lymphocyte homing/adhesion receptor;HERM;HERMES;hermes antigen;HUTCH-I;hyaluronate receptor;Ly-2;Ly-24;lymphocyte antigen 24;Pgp;Pgp-1;PGP-I;phagocytic glycoprotein 1;phagocytic glycoprotein I.
• NCBI Gene Id: 12505
• Protein Name: CD44 antigen
• Description of Target: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis (By similarity). Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (PubMed:25065622).
• Uniprot ID: P15379
• Protein Accession #: NP_001034240.1
• Nucleotide Accession #: NM_001039151.1