Catalog No: OKCD06644
Size:96WELLS
Price: $600.00
SKU
OKCD06644
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
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Datasheets/Manuals | Printable datasheet for CD44 ELISA Kit (Human) (OKCD06644) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Homing Associated Cell Adhesion Molecule (HCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Homing Associated Cell Adhesion Molecule (HCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Homing Associated Cell Adhesion Molecule (HCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Homing Associated Cell Adhesion Molecule (HCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 1.56-100ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Homing Associated Cell Adhesion Molecule (HCAM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Homing Associated Cell Adhesion Molecule (HCAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.56ng/mL |
Gene Symbol | CD44 |
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Gene Full Name | CD44 molecule (Indian blood group) |
Alias Symbols | CD44 antigen;CDW44;cell surface glycoprotein CD44;chondroitin sulfate proteoglycan 8;CSPG8;ECMR-III;epican;extracellular matrix receptor III;GP90 lymphocyte homing/adhesion receptor;HCELL;hematopoietic cell E- and L-selectin ligand;heparan sulfate proteoglycan;Hermes antigen;homing function and Indian blood group system;HUTCH-I;hyaluronate receptor;IN;Indian blood group antigen;LHR;MC56;MDU2;MDU3;MIC4;Pgp1;phagocytic glycoprotein 1;Phagocytic glycoprotein I;soluble CD44. |
NCBI Gene Id | 960 |
Protein Name | CD44 antigen |
Description of Target | Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity). |
Uniprot ID | P16070 |
Protein Accession # | NP_000601.3 |
Nucleotide Accession # | NM_000610.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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