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Catalog No: OKEH02681
Price: $475.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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CD44 ELISA Kit (Human) : 96 Wells (OKEH02681)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02681
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CD44 ELISA Kit (Human) (OKEH02681) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD44 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CD44 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD44 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
ELISA Kit Component
Anti-CD44 Microplate96 Wells (12 x 8 Well strips)
CD44 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CD44 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.045 ng/mL
SpecificityNatural and recombinant Human CD44 antigen
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCD44
Gene Full NameCD44 molecule (Indian blood group)
Alias SymbolsCD44 antigen;CDW44;cell surface glycoprotein CD44;chondroitin sulfate proteoglycan 8;CSPG8;ECMR-III;epican;extracellular matrix receptor III;GP90 lymphocyte homing/adhesion receptor;HCELL;hematopoietic cell E- and L-selectin ligand;heparan sulfate proteoglycan;Hermes antigen;homing function and Indian blood group system;HUTCH-I;hyaluronate receptor;IN;Indian blood group antigen;LHR;MC56;MDU2;MDU3;MIC4;Pgp1;phagocytic glycoprotein 1;Phagocytic glycoprotein I;soluble CD44.
NCBI Gene Id960
Protein NameP16070
Description of TargetCD44 antigen
Uniprot ID
Protein Accession #NP_000601.3
Nucleotide Accession #NM_000610.3
Protein Size (# AA)742
Molecular Weight82 kDa
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