CD44 Antibody - C-terminal region (OABB00945)

Catalog No: OABB00945

Size:100UG

Anti-CD44 antibody, OABB00945, Western blotting
All lanes: Anti CD44 (OABB00945) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 82 kDa
Observed bind size: 82 kDa

Anti-CD44 antibody, OABB00945, IHC(F)
IHC(F): Rat Intestine Tissue

Anti-CD44 antibody, OABB00945, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Anti-CD44 antibody, OABB00945, IHC(P)
IHC(P): Rat Intestine Tissue

Anti-CD44 antibody, OABB00945, ICC
ICC: HCT116 Cell

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Hamster|Human|Rat
• Product Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Flow cytometry|Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  Background: The CD44 gene, which is a transmembrane protein, is expressed as a family of molecular isoforms generated from alternative RNA splicing and posttranslational modifications. The gene, which contains 19 exons spanning some 50 kb of genomic DNA, is a widely expressed integral membrane protein that acts as a receptor for hyaluronan(HA) and is important to cell-extracellular matrix interaction. CD44 binding with HA can play an important role in cellular aggregation and tumor cell growth. CD44 is necessary for limb development and functions in a novel growth factor presentation mechanism. A specific CD44 splice variant is crucial for the proliferation of these mesenchymal cells. CD44 glycoproteins are involved in leukocyte extravasation but also in the regulation of growth factor activation, stability, and signaling. Moreover, it plays a pivotal role in arteriogenesis.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
  Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Rat: Human, Mouse
  Immunocytochemistry : 0.5-1 ug/ml: Human: Mouse, Rat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for CD44 antigen(CD44) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

Target Info

• Gene Symbol: CD44
• Gene Full Name: CD44 molecule (Indian blood group)
• Alias Symbols: CD44 antigen;CDW44;cell surface glycoprotein CD44;chondroitin sulfate proteoglycan 8;CSPG8;ECMR-III;epican;extracellular matrix receptor III;GP90 lymphocyte homing/adhesion receptor;HCELL;hematopoietic cell E- and L-selectin ligand;heparan sulfate proteoglycan;Hermes antigen;homing function and Indian blood group system;HUTCH-I;hyaluronate receptor;IN;Indian blood group antigen;LHR;MC56;MDU2;MDU3;MIC4;Pgp1;phagocytic glycoprotein 1;Phagocytic glycoprotein I;soluble CD44.
• NCBI Gene Id: 960
• Protein Name: CD44 antigen
• Description of Target: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity).
• Uniprot ID: P16070
• Molecular Weight: 81538 MW