CD40LG Antibody : PE (OATA00355)

Catalog No: OATA00355

Human PBMCs were stimulated with PMA + Ionomycin for 5 hours and then stained with 5 uL (0.25 ug) PE Anti-Human CD154 (OATA00355) (right panel) or 0.25 ug PE Mouse IgG2a isotype control (left panel).

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Rhesus monkey, cynomolgus monkey
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: 5C8
• Isotype: Mouse IgG2a, kappa
• Host: Mouse
• Conjugation: PE: Phycoerythrin
• Application: FC
  Excitation Laser: Blue (488 nm), Green (532 nm), Yellow-Green (561 nm)
  Emmision: 578 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD154 (CD40L)
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.

Target Info

• Gene Symbol: CD40LG
• Gene Full Name: CD40 ligand
• Alias Symbols: IGM, IMD3, TRAP, gp39, CD154, CD40L, HIGM1, T-BAM, TNFSF5, hCD40L
• NCBI Gene Id: 959
• Protein Name: CD40 ligand
• Description of Target: The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.