CD40L ELISA Kit (Mouse) (OKBB00633)

Catalog No: OKBB00633

Size:96 Wells

Mouse CD40L ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse CD40L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD40L has been precoated onto 96-well plates. Standards(NS0, E61-L260) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD40L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD40L amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	537	1562	3025	562	1675	3140
  SD	28.5	71.9	172.4	36.53	97.15	207.24
  CV%	5.3	4.6	5.7	6.5	5.8	6.6

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse CD40L Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse CD40L Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse CD40L Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse CD40L
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: E61-L260

Target Info

• Gene Symbol: CD40LG
• Gene Full Name: CD40 ligand
• Alias Symbols: CD154, CD40 antigen ligand, CD40 ligand, CD40-L, CD40L_HUMAN, CD40LG, gp39, HIGM1, IGM, IMD3, soluble form, T B cell activating molecule, T BAM, T cell antigen Gp39, T-cell antigen Gp39, TNF related activation protein, TNF-related activation protein, TNFSF5, TrAP, Tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), Tumor necrosis factor ligand superfamily member 5
• NCBI Gene Id: 21947
• Protein Name: CD40 ligand
• Description of Target: CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.
• Uniprot ID: P27548
• Protein Accession #: NP_035746.2
• Nucleotide Accession #: NM_011616.2