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Cd40 Antibody (OASA05250)

0.1 mg
$309.00
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Gene Symbol:
Cd40
NCBI Gene Id:
21939
Protein Name:
Tumor necrosis factor receptor superfamily member 5
Swissprot Id:
P27512
Protein Accession #:
NP_035741.2
Alias Symbols:
IGM, p50, Bp50, GP39, IMD3, TRAP, HIGM1, T-BAM, Tnfrsf5, AI326936, Cd40
Replacement Item:
This antibody may replace item sc-112948 from Santa Cruz Biotechnology.
Description of Target:
RAT ANTI MOUSE CD40
Protein Size (# AA):
289
Host:
Rat
Clonality:
Monoclonal
Application:
IHC-AFF, ELISA, FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Cd40.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Cd40.
Immunogen:
The immunogen for anti-Cd40 antibody: extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1
Predicted Species Reactivity:
Mouse
Predicted Homology Based on Immunogen Sequence:
Mouse
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-Cd40 antibody - OASA05250
Specificity:
CD40
Clone:
3/23
Isotype:
IgG2a
Application Info:
Flow Cytometry:    1/10 - 1/50
Application Data:
Application #1: Staining of mouse splenocytes with Rat anti Mouse CD40:RPE
Application #2: Staining of mouse spleen cells with Rat anti Mouse CD40
Application #3: Staining of mouse spleen cells with Rat anti Mouse CD40:Azide Free
Application #4: Staining of mouse spleen cells with Rat anti Mouse CD40:FITC
Application #5: Staining of mouse spleen with Rat anti Mouse CD40: Alexa Fluor 647
Additional Information:
Fusion Partners: Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
:::
Approx Protein Conc: IgG concentration 1 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Parry, S.L. et al. (1994) Plastic-immobilized anti-mu or anti-delta antibodies induce apoptosis in mature murine B lymphocytes. Eur. J. Immunol. 24: 974-979.
2. Hasbold, J. et al. (1994) Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies. Eur. J. Immunol. 24: 1835-1842.
3. Hasbold, J. and Klaus, G.G.B. (1994) B cells from CBA/N mice do not proliferate following ligation of CD40. Eur. J. Immunol. 24: 152-157.

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