CD40 Antibody : FITC (OATA00210)

Catalog No: OATA00210

Human peripheral blood lymphocytes were stained with APC Anti-Human CD19 (OATA00038) and 5 uL (0.5 ug) FITC Anti-Human CD40 (OATA00210) (right panel) or 0.5 ug FITC Mouse IgG1 isotype control (left panel).

Product Info

• Tested Species Reactivity: Human
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: G28.5
• Isotype: Mouse IgG1, kappa
• Host: Mouse
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: FC
  Excitation Laser: Blue (488 nm)
  Emmision: 520 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD40
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
• Reference:

  Clark EA, Ledbetter JA. 1986. Proc Natl Acad Sci U S A. 1986; 83(12):4494-4498.

  Zhang K, Clark EA, Saxon A. 1991. J Immunol. Mar 15;146(6):1836-42.

  Galy AH, Spits H. 1992. J Immunol. 149(3):775-782.

  Challa A, Pound JD, Armitage RJ, Gordon J. 1999. Allergy. Jun;54(6):576-83.

  Cognasse F, Chavarin P, Acquart S, Sabido O, Beniguel L, Genin C, Richard Y, Garraud O. 2005. Int J Immunopathol Pharmacol. Jan-Mar;18(1):65-74.

Target Info

• Gene Symbol: CD40
• Gene Full Name: CD40 molecule
• Alias Symbols: p50, Bp50, CDW40, TNFRSF5
• NCBI Gene Id: 958
• Protein Name: tumor necrosis factor receptor superfamily member 5
• Description of Target: This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.