Search Antibody, Protein, and ELISA Kit Solutions

Cd4 Antibody (OASA05216)

0.1 mg
$309.00
In Stock
Request Bulk Order Quote

Gene Symbol:
Cd4
NCBI Gene Id:
12504
Protein Name:
T-cell surface glycoprotein CD4
Swissprot Id:
P06332
Protein Accession #:
NP_038516.1
Alias Symbols:
L3T4, Ly-4, Cd4
Replacement Item:
This antibody may replace item sc-1140 from Santa Cruz Biotechnology.
Description of Target:
RAT ANTI MOUSE CD4
Protein Size (# AA):
457
Host:
Rat
Clonality:
Monoclonal
Application:
IHC-AFF, ELISA, FC, IF, IP, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Cd4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Cd4.
Immunogen:
The immunogen for anti-Cd4 antibody: murine CD4
Predicted Species Reactivity:
Mouse
Predicted Homology Based on Immunogen Sequence:
Mouse
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-Cd4 antibody - OASA05216
Specificity:
CD4
Clone:
GK1.5
Isotype:
IgG2b
Application Info:
Flow Cytometry:    1/10 - 1/200
Application Data:
Application #1: Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:Low Endotoxin
Application #2: Staining of mouse spleen with Rat anti Mouse CD4
Application #3: Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4 Alexa Fluor ?647
Application #4: Staining of mouse spleen with Rat anti Mouse CD4:Alexa Fluor ?488
Application #5: Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC
Additional Information:
Fusion Partners: Spleen cells from immunised Lewis rats were fused with cells of the SP2/0 myeloma cell line.
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Dialynas, D.P. et al. (1983) Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol.131:2445-2451
2. Wilde, D.B. et al. (1983) Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 131:2178-2183.
3. Dialynas, D.P. et al. (1983) Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 74:29-56.

Product Reviews

Protocol:
Tips Information:

Tell us what you think about this item!

Write A Review
    Please, wait...