Size:0.1 mg
$359.00
Discontinued
SKU
OASA05216
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More Information
Predicted Species Reactivity Mouse
Product Format Purified IgG - liquid
Clonality Monoclonal
Clone GK1.5
Isotype IgG2b
Host Rat
Application IHC-AFF, FC, IF, IP, WB
Additional Information Fusion Partners: Spleen cells from immunised Lewis rats were fused with cells of the SP2/0 myeloma cell line.
:: Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
:: Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Reconstitution and Storage Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Replacement Item This antibody may replace item sc-1140 from Santa Cruz Biotechnology.
Immunogen The immunogen for anti-Cd4 antibody: murine CD4
Predicted Homology Based on Immunogen Sequence Mouse
Datasheets/Manuals Printable datasheet for anti-Cd4 antibody - OASA05216
Specificity CD4
Application Info Flow Cytometry:    1/10 - 1/200
Application Data Application #1: Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:Low Endotoxin
Application #2: Staining of mouse spleen with Rat anti Mouse CD4
Application #3: Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4 Alexa Fluor ?647
Application #4: Staining of mouse spleen with Rat anti Mouse CD4:Alexa Fluor ?488
Application #5: Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC
Target Reference 1. Dialynas, D.P. et al. (1983) Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol.131:2445-2451
2. Wilde, D.B. et al. (1983) Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 131:2178-2183.
3. Dialynas, D.P. et al. (1983) Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 74:29-56.
Gene Symbol Cd4
Alias Symbols L3T4, Ly-4, Cd4
NCBI Gene Id 12504
Protein Name T-cell surface glycoprotein CD4
Description of Target RAT ANTI MOUSE CD4
Swissprot Id P06332
Protein Accession # NP_038516.1
Protein Size (# AA) 457
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Cd4.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Cd4.
  1. What is the species homology for "Cd4 Antibody (OASA05216)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse".

  2. How long will it take to receive "Cd4 Antibody (OASA05216)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "Cd4 Antibody (OASA05216)" provided in?

    This item is provided in "Purified IgG - liquid ".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Cd4 Antibody (OASA05216)"?

    This target may also be called "L3T4, Ly-4, Cd4" in publications.

  5. What is the shipping cost for "Cd4 Antibody (OASA05216)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Cd4 Antibody (OASA05216)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Cd4 Antibody (OASA05216)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Cd4 Antibody (OASA05216)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "CD4"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CD4"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CD4"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CD4"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CD4"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CD4"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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