- NCBI Gene Id:
- Protein Name:
- T-cell surface glycoprotein CD4
- Alias Symbols:
- L3T4, Ly-4, Cd4
- Replacement Item:
- This antibody may replace item sc-1140 from Santa Cruz Biotechnology.
- Description of Target:
- RAT ANTI MOUSE CD4
- Protein Size (# AA):
- IHC-AFF, FC
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express Cd4.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express Cd4.
- Predicted Species Reactivity:
- Predicted Homology Based on Immunogen Sequence:
- Product Format:
- Purified IgG - liquid
- Reconstitution and Storage:
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for anti-Cd4 antibody - OASA05212
- Application Info:
- Flow Cytometry: 1/50 - 1/100
- Application Data:
- Application #1: Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4: Alexa Fluor 405
Application #2: Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC
Application #3: Staining of mouse spleen cells with Rat anti Mouse CD4: APC
Application #4: Staining of mouse spleen cells with Rat anti Mouse CD4
Application #5: Staining of mouse spleen cells with Rat anti Mouse CD4:RPE
- Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
- Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
- Target Reference:
- 1. Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in MHC-mismatched or primed recipients: primed T cells can be tolerized in the periphery with CD4 and CD8 antibodies. Eur. J. Immunol. 20: 2747-2755.
2. Bemelman, F. et al. (1998) Bone marrow transplantation induces either clonal deletion or infectious tolerance depending on the dose. J. Immunol. 160: 2645-2648.
3. Higgins, L. M. et al. (1999) Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 Ligand Interaction: Amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand - IgG fusion protein. J. Immunol. 162: 486 - 493.
- Tips Information: