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Cd4 Antibody (OASA03956)

0.1 mg
$309.00
In Stock
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Gene Symbol:
Cd4
NCBI Gene Id:
24932
Protein Name:
T-cell surface glycoprotein CD4
Swissprot Id:
P05540
Protein Accession #:
NP_036837.1
Alias Symbols:
p55, W3/25, Cd4
Replacement Item:
This antibody may replace item sc-1140 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI RAT CD4 (DOMAIN 1)
Protein Size (# AA):
457
Host:
Mouse
Clonality:
Monoclonal
Purification:
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Application:
IHC-P, IHC-F, FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Cd4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Cd4.
Immunogen:
Rat Thymocyte Membrane Glycoproteins
Predicted Species Reactivity:
Rat
Predicted Homology Based on Immunogen Sequence:
Rat
Product Format:
Liquid. PBS with 0.09% (W/V) sodium azide.
Reconstitution and Storage:
Store at +4C for one month or at -20C for longer.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch.
Datasheets/Manuals:
Printable datasheet for anti-Cd4 antibody - OASA03956
Specificity:
Mouse anti Rat CD4 antibody, clone W3/25 recognizes the rat CD4 cell surface glycoprotein. This antibody inhibits proliferation and IL-2 production in the MLR reaction.
Clone:
W3/25
Isotype:
IgG1
Application Info:
FC 1/100 - 1/200; Use 10ul of the suggested working dilution to label 106 cells in 100ul.
IHC-P PLP fixation is recommended for optimal results, see Whiteland et al. for details
Additional Information:
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the NS-1 mouse myeloma cell line.
Target Reference:
1. Williams, A.F. et al. (1977) Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 12: 663-673.
2. Barclay, A.N. (1981) The localization of populations defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 42: 593-600.
3. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320.
4. Pelegr, C.et al. (1995) Immunohistochemical changes in synovial tissue during the course of adjuvant arthritis. J. Rheumatol. 22: 124-132.
5. Hofmann, N. et al. (2002) Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats. BMC Immunol. 3: 11.
6. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and

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