CD3G Antibody (OAGA03906)

Catalog No: OAGA03906

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Molt-4
12% SDS PAGE
Diluted at 1:3000

CD3 gamma antibody [C1C3] detects CD3 gamma protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human PANC1 xenograft.
CD3 gamma antibody [C1C3] diluted at 1:500.

CD3 gamma antibody [C1C3] detects CD3 gamma protein by flow cytometry analysis.
Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4C for 15 min.
Black: Unlabelled sample was used as a control.
Red: CD3 gamma antibody [C1C3] dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: FACS, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD3 gamma. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: FACS: 1:50-1:200, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: CD3G
• Gene Full Name: CD3g molecule
• Alias Symbols: T3G, IMD17, CD3-GAMMA
• NCBI Gene Id: 917
• Protein Name: T-cell surface glycoprotein CD3 gamma chain
• Description of Target: The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.
• Uniprot ID: P09693
• Protein Accession #: NP_000064.1
• Nucleotide Accession #: NM_000073.2