CD3E ELISA Kit (Human) : 96 Wells (OKEH01335)

Catalog No: OKEH01335 (Formerly GWB-HD6E6H)

Size:96T

Human CD3E ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CD3E ELISA Kit (Human) (OKEH01335) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD3E has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD3E is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD3E captured in well.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.2% (n = 20)
  Mean Inter-assay CV%: < 9.6% (n = 20)
• ELISA Kit Component:
  • CD3E Microplate: 96 Wells (12 x 8 Well strips)
  • CD3E Lyophilized Standard: 2
  • 100X Biotinylated CD3E Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.23 ng/mL
• Specificity: Natural and recombinant Human T-cell surface glycoprotein CD3 epsilon chain
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CD3E
• Gene Full Name: CD3e molecule
• Alias Symbols: CD3e antigen, epsilon polypeptide (TiT3 complex);CD3e molecule, epsilon (CD3-TCR complex);CD3-epsilon;IMD18;T3E;T-cell antigen receptor complex, epsilon subunit of T3;T-cell surface antigen T3/Leu-4 epsilon chain;T-cell surface glycoprotein CD3 epsilon chain;TCRE.
• NCBI Gene Id: 916
• Protein Name: P07766
• Description of Target: T-cell surface glycoprotein CD3 epsilon chain
• Uniprot ID: https://www.uniprot.org/uniprot/P07766
• Protein Accession #: NP_000724.1
• Nucleotide Accession #: NM_000733.3
• Protein Size (# AA): 207
• Molecular Weight: 23 kDa