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Datasheets/Manuals | Printable datasheet for Cd3e Antibody (Phospho-Tyr72) (OAEE00196) (OAEE00196) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Clonality | Monoclonal |
Clone | EM-26 |
Isotype | IgG2b |
Host | Mouse |
Application | Flow cytometry|Immunocytochemistry|Western blot |
Reconstitution and Storage | 2°C to 8°C |
Immunogen | A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH |
Purification | Purified |
Concentration | 1 mg/ml |
Specificity | The mouse monoclonal antibody EM-26 recognizes phosphorylated tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells. |
Purity | > 95% (by SDS-PAGE) |
Application Info | FC: 1-9 ug/mL. Positive Control: Jurkat cells treated with pervanadate; T-cells from lymph nodes of OT-1 mouse treated with pervanadate. WB: 2-5 ug/mL. Positive Control: Jurkat cell lysate treated with pervanadate; Splenocyte lysate of Balb/c or F1 mouse treated with pervanadate. Non-reducing conditions recommended |
Reference | Borroto A, Arellano I, Dopfer EP, Prouza M, Suchànek M, Fuentes M, Orfao A, Schamel WW, Alarcón B: Nck recruitment to the TCR required for ZAP70 activation during thymic development. J Immunol. 2013 Feb 1;190(3):1103-12. Dopfer EP, Schöpf B, Louis-Dit-Sully C, Dengler E, Höhne K, Klescová A, Prouza M, Suchanek M, Reth M, Schamel WW: Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR-CD3 signalling. Immunol Lett. 2010 May 4;130(1-2):43-50 |
Gene Symbol | CD247 |
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Gene Full Name | CD247 molecule |
Alias Symbols | CD247 antigen, zeta subunit;CD3H;CD3Q;CD3Z;CD3Z antigen, zeta polypeptide (TiT3 complex);CD3-ZETA;CD3zeta chain;IMD25;T3Z;T-cell antigen receptor complex, zeta subunit of CD3;T-cell receptor T3 zeta chain;T-cell surface glycoprotein CD3 zeta chain;TCR zeta chain;TCRZ. |
NCBI Gene Id | 919 |
Protein Name | T-cell surface glycoprotein CD3 zeta chain |
Description of Target | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083). CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083). Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity). |
Uniprot ID | P20963 |
Protein Accession # | NP_031674.1; XP_006510029.1 |
Nucleotide Accession # | NM_007648.4; XM_006509966.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Cd3e Antibody (Phospho-Tyr72) (OAEE00196)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "Cd3e Antibody (Phospho-Tyr72) (OAEE00196)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Cd3e Antibody (Phospho-Tyr72) (OAEE00196)" provided in?
This item is provided in "Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Cd3e Antibody (Phospho-Tyr72) (OAEE00196)"?
This target may also be called "CD247 antigen, zeta subunit;CD3H;CD3Q;CD3Z;CD3Z antigen, zeta polypeptide (TiT3 complex);CD3-ZETA;CD3zeta chain;IMD25;T3Z;T-cell antigen receptor complex, zeta subunit of CD3;T-cell receptor T3 zeta chain;T-cell surface glycoprotein CD3 zeta chain;TCR zeta chain;TCRZ." in publications.
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What is the shipping cost for "Cd3e Antibody (Phospho-Tyr72) (OAEE00196)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Cd3e Antibody (Phospho-Tyr72) (OAEE00196)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Cd3e Antibody (Phospho-Tyr72) (OAEE00196)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Cd3e Antibody (Phospho-Tyr72) (OAEE00196)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CD247"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CD247"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CD247"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CD247"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CD247"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CD247"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.