- NCBI Gene Id:
- Protein Name:
- T-cell surface glycoprotein CD3 epsilon chain
- Alias Symbols:
- T3E, TCRE, FLJ18683, CD3E
- Replacement Item:
- This antibody may replace item sc-1127 from Santa Cruz Biotechnology.
- Description of Target:
- RAT ANTI HUMAN CD3:FITC
- Protein Size (# AA):
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express CD3E.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express CD3E.
- The immunogen for anti-CD3E antibody: synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys)
- Predicted Species Reactivity:
- Predicted Homology Based on Immunogen Sequence:
- Product Format:
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Reconstitution and Storage:
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for anti-CD3E antibody - OASA04636
- Application Info:
- Flow Cytometry(1): 1/5 - 1/10
- Application Data:
- Application #1: Staining of human peripheral blood lymphocytes with Rat anti Human CD3 following permeabilisation with Leucoperm (BUF09)
Application #2: Staining of human peripheral blood lymphocytes with Rat anti Human CD3 . Permeabilised with Leucoperm (BUF09)
Application #3: Staining of human peripheral blood lymphocytes with Rat anti Human CD3:RPE . Permeabilised with Leucoperm (BUF09)
- Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
1% - Bovine Serum Albumin
- Approx Protein Conc: IgG concentration 0.1 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
- Target Reference:
- 1. Jones, M. et al. (1993) Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J. Immunol. 150: 5429-5435.
2. Alterio de Goss, M. et al. (1998) Control of cytomegalovirus in bone marrow transplantation chimeras lacking the prevailing antigen-presenting molecule in recipient tissues rests primarily on recipient- derived CD8 T cells. J. Virol. 72: 7733 - 7744.
3. Burudi, E. M. et al. (2002) Regulation of indoleamine 2, 3-dioxygenase expression in Simian Immunodeficiency Virus-infected monkey brains. J. Virol. 76: 12233 - 12241.
4. Shulga Morskaya, S. et al. (2004) B Cell-Activating Factor Belonging to the TNF Family Acts through Separate Receptors to Support B Cell Survival and T Cell-Independent Antibody Formation. J. Immunol. 173: 2331-2341.
5. Kapturczak, M. H. et al. (2004) Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse. Am. J. Pathol. 165: 1045-1053.
6. Kothlow, S. et al. (2005) Characterization of Duck Leukocytes by monoclonal antibodies. Dev. & Comp. Immunology 29: 733 -748.
- Tips Information: